Method For Performing Quantitation Assays

进行定量测定的方法

Abstract

本发明涉及用于确定分析物分子或粒子的浓度估值E(C)的方法,其中将预定体积的样品分至一定数目的(N)隔室,所述(N)隔室包含不同样品体积(vi)和/或不同稀释因数(di)的样品或由其组成,在所述(N)隔室的任一个中存在的至少部分分子或粒子提供可测量的信号并且所述分子或粒子的估计浓度E(C)为所测量信号的函数,以及涉及用于本发明的方法的装置,本发明方法的应用,在用于本发明方法样品架和试剂盒。
The present invention relates to a method for determining an estimate of a concentration of analyte molecules or particles E(C), wherein a sample of predetermined volume is divided into a number (N) of compartments, the (N) compartments comprise or consist of different sample volumes (Vi) and/or different dilution factors (di) of the sample, at least part of the analyte molecules or particles that are present in any of the (N) compartments provide a measurable signal and the estimated concentration of molecules or particles E(C) is a function of measured signals, as well as an apparatus for use in the inventive method, uses of the inventive method, a sample holder and a kit for use in the inventive method.

Claims

Description

Topics

Download Full PDF Version (Non-Commercial Use)

Patent Citations (0)

    Publication numberPublication dateAssigneeTitle

NO-Patent Citations (2)

    Title
    COCHRAN W G等: "Estimation of bacterial densities by means of the "most probable number"", 《BIOMETRICS》
    JASON E. KREUTZ等: "Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR", 《ANALYTICAL CHEMISTRY》

Cited By (0)

    Publication numberPublication dateAssigneeTitle